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KMID : 1007520110200010151
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Food Science and Biotechnology
2011 Volume.20 No. 1 p.151 ~ p.158
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Catechin-7-O-β-D-Glucopyranoside Scavenges Free Radicals and Protects Human B Lymphoma BJAB Cells on H2O2-mediated Oxidative Stress
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Baek Jin-A
Son Young-Ok
Fang Ming-hao
Lee Young-Jae
Cho Hyoung-Kwon
Whang Wan-Kyunn
Lee Jeong-Chae
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Abstract
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Catechin-7-O-β-D-glucopyranoside (CA-G) was previously isolated from red bean (the seed of Phaseolus calcaratus cv. Roxburgh). This study examined the ability of CA-G to scavenge reactive oxygen species generated by cell-free systems and to protect cells from oxidative stress caused by hydrogen peroxide (H2O2). The mechanism by which CA-G exerts its antioxidant and anti-apoptotic action on H2O2-exposed cells was also investigated. CA-G treatment prevented H2O2-mediated apoptosis and inhibited the formation of single stand breaks in DNA in H2O2- exposed BJAB cells. CA-G suppressed mitochondrial stress and caspase activation caused by H2O2. Mechanistic experiments revealed that the antioxidant mechanism of CA-G on H2O2-mediated oxidative damage was due to the direct scavenging of hydroxyl radicals and/or to the chelation of metal ions that were used to produce hydroxyl radicals from H2O2 via the Fenton reaction. Collectively, these findings suggest beneficial roles of CA-G or CA-Grich red bean on the protection from oxidative damage.
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KEYWORD
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apoptosis, catechin-7-O-β-D-glucopyranoside, DNA damage, hydrogen peroxide, oxidative stress, red bean
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